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mouse monoclonal anti nka α subunit antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse monoclonal anti nka α subunit antibody
    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
    Mouse Monoclonal Anti Nka α Subunit Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 964 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti nka α subunit antibody/product/Developmental Studies Hybridoma Bank
    Average 95 stars, based on 964 article reviews
    mouse monoclonal anti nka α subunit antibody - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "The mechanism of action of digoxin requires the sodium-dependent inactivation of the sodium-calcium exchanger"

    Article Title: The mechanism of action of digoxin requires the sodium-dependent inactivation of the sodium-calcium exchanger

    Journal: Science Advances

    doi: 10.1126/sciadv.ady9596

    ( A ) Representative Western blot of NKA α-subunit (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
    Figure Legend Snippet: ( A ) Representative Western blot of NKA α-subunit (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.

    Techniques Used: Western Blot, Expressing, Immunofluorescence, Staining, Fluorescence



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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
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    Average 90 stars, based on 1 article reviews
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    ( A ) Representative Western blot of <t>NKA</t> <t>α-subunit</t> (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.
    Mouse Monoclonal Anti Nka A1 Subunit Antiserum, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Representative Western blot of NKA α-subunit (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.

    Journal: Science Advances

    Article Title: The mechanism of action of digoxin requires the sodium-dependent inactivation of the sodium-calcium exchanger

    doi: 10.1126/sciadv.ady9596

    Figure Lengend Snippet: ( A ) Representative Western blot of NKA α-subunit (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.

    Article Snippet: Myocytes were then incubated with the mouse monoclonal anti-NKA α-subunit antibody (1:200, a5, DSHB) in 3% BSA PBS overnight at 4°C.

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Fluorescence